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In this experiment, students prepare a protein extract from cow heart. They then
determine the molecular weights of major contractile proteins by comparing their
migration on SDS-polyacrylamide gels to the migration of standard proteins of
known size. Students also identify and determine the molecular weight of the
major proteins found in milk. This exercise requires 1 three-hour or 2 two-hour
lab sessions and a table top centrifuge or microcentrifuge is required. Typical
results of the exercise are shown on the gel at the right. Includes cow heart,
milk, standard proteins, sample buffer, and instructions. Requires four 12 well
gels for 8 groups of students or two gels for 6 groups. Click Here for polyacryamide electrophoresis packages and gels.
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