Purified proteins are often needed in the basic research laboratory and for
diagnostic and therapeutic procedures. An effective technique for protein
purification is affinity chromatography, which exploits a specific interaction
between a protein and a complementary binding molecule. In this exercise,
students isolate albumin from horse serum by affinity chromatography using a
column matrix containing a reactive blue dye, which binds specifically to the
albumin molecule. They then use electrophoresis to analyze the isolated protein
in order to verify the effectiveness of the procedure. Includes Affi-gel Blue,
chromatographic columns, column buffer, elution buffer, standard proteins, horse
serum and instructions. Requires four 12 well gels for 8 groups of students or
two gels for 6 groups.
Click Here for polyacryamide
electrophoresis packages and gels.
Modern
Biology Inc