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Chromatographic Separation of Proteins (B3)


Gel filtration is a chromatographic method used to separate molecules of different sizes. This technique is an important tool for isolating and characterizing proteins. In this series of experiments, students use this method along with basic enzyme and antibody assays to study the structure and function of proteins.

B3 1-3 Mini Program in Chromatography (View Individual Experiment)

1. Separating Molecules by Gel Filtration

Students fill their chromatographic columns with packing material and separate three colored molecules of known molecular weights as shown in the photograph below. The exercise illustrates how components of a complex mixture can be separated by chromatography and introduces the concept of the standard curve for determining the size of an unknown protein.

2. The Molecular Weight of Hemoglobin
Students determine the size of hemoglobin and estimate the number of amino acid residues in this protein.

B3-4 Properties of Amylase (View Individual Experiment)

Gel filtration chromatography has the advantage of being able to yield the size of different proteins in a complex mixture. This feature is illustrated in this experiment where students compare the molecular weight of amylase from pancreas and saliva.

B3-5 Analysis of a Protein From Egg White. (View Individual Experiment)

Students first isolate ovalbumin, a major protein found in egg white. They then use gel filtration and a simple immunological procedure to determine the size of this protein and the amount of it in the egg.

Prices

Catalog # Price Description
B3 263.82

The Chemical Package for 16 students working in pairs plus 17 student manuals and one instructor manual.
B3-C 207.99

The Chemical Package for 16 students working in pairs plus one student manual and one instructor manual (The student manual may be reproduced for educational purposes.)
B3-CR 146.86

B3-CR Chemical Refill Package (contains the chemicals and biologicals only)
B3-SM 6.87

Sample Student Manual (43 pages) plus one instructor manual.

Basic Program 3 contains:

  • 8 Collection Plates
  • 8 Chromatographic Columns (column tubes, reservoirs, lower fittings, bed supports and caps)
  • Rabbit Blood
  • 70 Small Test Tubes
  • Bromophenol Blue
  • 60 Transfer Pipets
  • Cow Serum Albumin(2)
  • 20 Petri Dishes
  • Agar
  • 2 Macropipetors
  • Column Packing Material
  • Starch Solution
  • 16 Large Test Tubes
  • Column Buffer
  • Potassium Iodine
  • 20 Pasteur Pipets
  • Phenol Red
  • Amylase
  • 8 Tube racks
  • Myoglobin-Blue Dextran
  • Pancreatic Extract
  • Cheese cloth
  • Cytochrome C-Hemoglobin
  • Ovalbumin
  • SDS
  • Lysis Buffer
  • Ovalbumin Antibodies