A Computer Course in DNA and RNA Sequence Analysis. (CDP-1)

This course introduces the student to the use of computers as research tools for analyzing nucleic acid and protein sequences. In the course, the student learns to operate three analytical programs in the detailed study of the structure and function of bacterial genes, a random sequence (generated by the student), the human ß-globin gene, and the entire genome of the human immunodeficiency virus, the causative factor of AIDS. Although the three programs that form the basis of the software package were written for the research laboratory, they are presented here in a form that is ideal for teaching situations. The manual provides the student with step-by-step instructions on how to use each program, an overview of the nature of nucleic acid sequences, and some 40 exercises and problems which provide extensive training in the following:

• Entering and modifying sequences from the keyboard, and printing sequences to the screen and/or line printer.

• Locating and analyzing protein coding regions and associated regulatory signals in nucleic acid sequences.

• Translating nucleic acid sequences into amino acid sequences for output to screen and/or line printer.

• Identifying restriction enzyme sites and other special regions in DNA, and employing restriction sites in the design of cloning strategies.

The course includes a manual and a CD.