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Bacterial Strains E. coli - Contains no PlasmidThis culture is supplied with directions for propagation. The cells can be transformed to an ampicillin-resistant phenotype with plasmid pUC-18.
E.coli-pUC18 - Contains plasmid pUC18 The cells have been transformed with pUC18.
Culture Media and Bacteria The prepared bacterial media listed below are provided sterile and are ready to use. Nutrient Broth This rich media is commonly used for the maintenance and propagation of E. coli.
Nutrient Broth + Ampicillin Selects for the ampicillin-resistant phenotype.
Nutrient Agar Nutrient agar for isolation and propagation of E. coli colonies. To prepare plates, simply melt the agar in the bottle in a water bath, cool and pour into 20 petri dishes.
a Nutrient Agar + AmpicillinSelects for the ampicillin-resistant phenotype.
a Nutrient Agar + Ampicillin + X galSelects for the ampicillin-resistant phenotype. Appropriate cells containing pUC18 ( such as those under cat.# 4-5-P) grow as blue colonies on agar-ampicillin-X gal plates.
Sterile Disposables for Bacterial Propagation Culture dishes The sterile polystyrene dishes hold 20-25 ml of media and are for growing bacterial colonies.
Culture Tubes These individually wrapped polystyrene tubes hold 19 ml of media. The sterile screw-cap tubes can be used for small (5-10 ml) overnight suspension cultures of bacteria.
Sterile Transfer Pipets The 3 ml individually wrapped pipets are self-filling by using an attached bulb. Ideal for transferring sterile media.
Inoculating Loops These sterile loops are made from polystyrene and hold 10 µl.
Micro-Test Tubes These clear polypropylene tubes are ideal for small reactions and the 1.5 ml size is also a microcentrifuge tube.
Micro-Tube Racks The polyurethane foam racks will float in a water bath and hold 15 - 0.5 ml or 1.5 ml micro-test tubes.
Large Micro-Tube Racks The polypropylene racks will hold up to 80 - 1.5 ml micro-test tubes.
Transfer Pipets These polyethylene pipets are self- filling by using an attached bulb. They are convenient for liquid chores and the 1 ml size can even be used to load agarose gels.
Reaction Plates These 24-well polystyrene plates are ideal for biochemical reactions. Each well is numbered and holds about 3 ml.
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