Modern
Biology Inc
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1-800-733-6544
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1. Laboratory Manuals
2. Chemical Package Essentially all of the reagents required to perform electrophoresis and to conduct each experiment are found in the chemical packages. We have avoided using known toxic or carcinogenic chemicals (such as: chloroform, ethidium bromide or acrylamide) and have replaced them with nontoxic substitutes. The components in each chemical mixture are listed in the instructor manuals which also contain an appendix on safety precautions. The chemical packages are shipped on ice and can be used for at least six months when properly stored.
3. Accessories
The procedures used in the programs are performed with the accessories in the Accessory Kit. The accessories are used for sample handling and for preparation of electrophoretic gels. Although you may have some of these items in your teaching laboratory, we recommend that you purchase the kit because substituted items may not be suitable. The same Accessory Kit is used for each program and the accessories in a single kit can be reused on a number of occasions. The microliter dispensers are also available individually as described on page 15 of this catalog.
4. Electrophoresis Equipment
Electrophoresis is one of the most important tools available to the molecular biologist for separating mixtures of proteins, RNA, and DNA. Electrophoresis is also used in our programs as a principle technique because it is versatile, relatively inexpensive, and easy to perform. In electrophoresis, charged molecules such as proteins or DNA are loaded into wells made in a sponge-like gel using the microliter dispenser as shown at the left. Agarose gels are used in the programs because they are prepared easily from nontoxic materials and can resolve both DNA and protein mixtures. Gels are placed in an electric field in the PROCELL electrophoresis unit described on the following page and the molecules move through the gel pores and separate according to their size or charge. Four agarose gels are run simultaneously using the PROCELL unit and MB-170 Power Supply (Electrophoresis running time: 45-60 minutes). After electrophoresis, gels are stained and viewed on a light box. |
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Clearly
written laboratory manuals are the key to the effective utilization of the
programs. Each manual provides information on the biochemistry of proteins or
nucleic acids and also acquaints students with electrophoresis theory and
techniques. Background information and clearly outlined procedures are also
given for each experiment. The average length of the manuals is 50 pages and
each is well illustrated. The instructor manuals contain answers to questions
posed in the student manuals as well as illustrations of the results of each
experiment. 
