102. Genetics and Sickle Cell Anemia (EXP-102)
In this analysis, hemoglobin from a normal individual (gel lanes 1, 4, 5, 8 ) is compared to hemoglobin S (lanes 3 & 7) and hemoglobin from a person who has sickle cell trait (lanes 2, & 6). Note the two forms of hemoglobin in the individual with sickle cell trait.
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Many changes in the structure of hemoglobin have arisen by mutations. About one person in 100 carries a mutant hemoglobin gene, and these individuals have abnormal hemoglobin molecules in their blood. One of the most common abnormal hemoglobins is hemoglobin S, which causes sickle cell anemia. When the gene for hemoglobin S is inherited from both parents, all of the hemoglobin in the circulation is hemoglobin S and the individual suffers from severe anemia. When the gene for hemoglobin S is inherited from only one parent, the individual is heterozygous for the condition and has sickle cell trait. Although these individuals rarely have severe anemia, half of their hemoglobin is hemoglobin S and half is normal hemoglobin. In this exercise, students compare the electrophoretic patterns of hemoglobin from a normal individual to hemoglobin S and hemoglobin from a person with sickle cell trait. Students are also given the opportunity to study their own hemoglobin in order to test for hemoglobin variants. Typical results of this exercise are shown.
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All online orders of this experiment placed between Aug 20, 2016 - Aug 27, 2016 will automatically receive 5 grams of electrophoresis grade agarose, and 25 grams of a Hemoglobin electrophoresis buffer - Enough to make 4L of a 9.2 TG buffer.
Please note that Exp-102 requires a TG buffer with a pH of 9.2
Electrophoresis package 1/8 provides sufficient agarose, buffers, and stains for this and 5 more experiments!)