105. A Comparison of Human and Bacterial Amylase (Exp-105)
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A powerful technique used in the modern laboratory involves the detection of specific enzymes in agarose gels following electrophoresis of proteins from serum, tissue extracts and other biological preparations. This procedure makes it possible to study certain properties of individual enzymes in complex protein mixtures. In this exercise, students are introduced to this procedure by comparing the amylase found in bacteria with that in their own saliva.
Electrophoresis package 1/8 provides sufficient agarose, buffers, and stains for this and 5 more experiments from this series!)