Modern
Biology Inc
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Modern Biology Inc. provides a range of individual products and kits for DNA and protein analysis that meet the stringent requirements of the research laboratory. The items indicated by the "a" are shipped next-day air on ice. Please include all such reagents on the same order to avoid excess shipping costs. Products for Protein Analysis Native (nondenaturing) Agarose Gel Electrophoresis Reagent Package-Native Protein Gels Chemical package and instructions for extracting proteins from tissues and for separating proteins using native agarose gel electrophoresis. Contains all reagents needed to separate nondenatured proteins by electrophoresis on 35 small agarose gels. Includes protein extraction buffer (enough to make 500ml), electrophoresis buffer (Tris-Glycine pH 8.6; enough to make 7 liters), agarose (10 g), gel loading buffer (5 ml of a 2 X solution), Coomassie Blue gel stain (enough to make one liter) and a mixture of three colored dyes that can be used to monitor an electrophoretic run.
Agarose Protein electrophoresis grade
Electrophoresis Buffer (Tris-Glycine, pH 8.6)
Hemoglobin Electrophoresis Buffer (Tris-Glycine, pH 9.2)-for separation of different hemoglobin types.
Gel-Loading Buffer (Glycerol-Bromophenol blue)
Coomassie Blue R Gel Stain
Dye Mixture A mixture of xylene cyanol, bromophenol blue and orange G that can be used to monitor the course of an electrophoretic run.
Protein Extraction Buffer The buffer is used to prepare tissue proteins for native agarose gel electrophoresis.
Electrophoresis Standard Proteins A colorful mixture of rabbit hemoglobin, bovine serum albumin, and bromophenol blue that can be used for calibrating nondenaturing protein gels.
LDH Substrate Substrate for detection of lactate dehrdogenase (LDH) isoenzymes in agarose gels.
SDS (denaturing) Agarose Gels Reagent Package-SDS Protein Gels Complete chemical package for extracting proteins from tissues and for separating proteins using SDS agarose gels. Contains all reagents needed to separate denatured proteins by electrophoresis on 30 small agarose gels. Includes protein extraction buffer (enough to make 200ml), electrophoresis buffer (Tris-Acetate-SDS; enough to make 6 liters), gel buffer (enough to make 500 ml), agarose (25 g), loading buffer (5 ml of a 2 X solution), and Coomassie Blue gel stain (enough to make one liter). The reagent package is also supplied with instructions for protein extraction and for preparation, electrophoresis and staining of agarose gels.
Agarose SDS-protein electrophoresis grade. A unique blend that permits formation of high concentration (6-7%) gels.
Electrophoresis Buffer (Tris-Acetate-SDS pH 8.3)
Gel Buffer (Tris-Borate-SDS pH 8.6)
Gel-Loading Buffer Contains Bromophenol Blue, Glycerol and SDS.
Coomassie Blue R Gel Stain
Protein Extraction Buffer The buffer is used to prepare tissue proteins for SDS gels.
Electrophoresis Standard Proteins A pre-stained mixture of myoglobin, ovalbumin, bovine albumin monomer, bovine albumin dimer used for calibrating SDS-agarose or SDS-polyacrylamide gels.
a Antibody-Antigen KitsThe two kits described below contain antigen, antibody (as antiserum), agarose, petri dishes, Pasteur pipet sample well formers and buffer for double diffusion analysis. Kit 1 Ovalbumin (Egg Albumin)-Anti-Ovalbumin Complete with 1 ml of antiserum (made in rabbits), 1 ml of chicken ovalbumin (50 mg), 1 ml of chicken serum and instructions for Ouchterlony double diffusion analysis. With this kit, you can determine the amount of ovalbumin in an egg or compare the ovalbumin in eggs from different avian species.
Kit 2 Cow Serum-Anti Cow Serum Albumin Complete with 1 ml of cow serum, 1 ml of antiserum, 1 ml of chicken serum and instructions for Ouchterlony double diffusion analysis.
Column Chromatography Column Chromatography Kit Contains all materials needed for 8 groups of students to separate hemoglobin from phenol red by gel filtration chromatography. Includes 8 columns, Sephadex G-100(7g), column buffer (enough to make 5 liters) column loading pipets, and enough phenol red and bovine hemoglobin for at least forty chromatographic runs.
Chromatographic Columns Each polypropylene column (8mm by 200mm; 9 ml bed volume) comes with a 20 ml reservoir, lower fitting, porous polyethylene bed support and cap (see pictures on page 6 ).
Sephadex G-100 A gel filtration chromatographic media typically used for separation of proteins that are between 5,000-100,000 daltons. The 7g hydrates to about 120 ml of column bed volume.
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