Modern
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This Program is a complete laboratory course for teaching cell biology or introductory molecular biology at the college level. The program is designed for 16 students working in pairs and provides essentially all of the chemicals and instructions that are needed to teach sixteen 2-3 hour laboratory sessions. The course consists of 14 experiments carefully selected from Standard Programs 1, 2, 3, and 7 that are presented in a comprehensive integrated laboratory manual. The Table of Contents of the manual and the catalog numbers of the experiments provided with the program are shown below. To use this program, you will need an Accessory Kit, the appropriate electrophoresis equipment, microscopes, and a water bath. A small centrifuge is required for 2 experiments. The price of the course is about $51 per student per semester if these items are available in your teaching laboratory. In this laboratory course, your students will be introduced to the molecular biology of the eukaryotic cell. In the first section of the course, students study topics in protein biology and biochemistry such as protein structure, function, isolation, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis (both native and denaturing), affinity chromatography, peptide mapping, and the Western blot procedure. In the second section of the course, students localize enzymes in plant and animal cells, perform cell fractionation procedures, and study the properties of a specific cell-surface receptor. Experiments on the properties and structure of DNA are presented in the final section of the course. Techniques include DNA electrophoresis and restriction nuclease mapping. Features:
The experiments that comprise this course were selected from four Programs: Program Experiment Standard 1 101, 102, 106 Standard 2 201, 204, 205, 206 Standard 3 301, 302, 303, 306 Standard 7 701, 702, 703 Descriptions of the experiments and chemicals are given under the individual program descriptions. Price List - Laboratory Course 2
Table of Contents Part 1. The Proteins I. Protein Composition and Structure: A Review of the Basic.................................1II. Electrophoresis-General Information and Analysis of Native Proteins Theoretical Aspects..........................................................................................7 Practical Aspects and Procedures.......................................................................11 Experimental Analysis I 1. Electrophoretic Separation of Proteins.................................................. 17 2. Genetics and Sickle Cell Anemia.......................................................... .20 3. LDH Isoenzymes....................................................................................26 III. Electrophoretic Analysis of Denatured Proteins General Information...................................................................................32 Experimental Analysis II 4. Molecular Weight Determination..........................................................36 5. Peptide Mapping Analysis.....................................................................40 6. Protein Evolution and the Western Blot................................................44 7. Affinity Chromatography......................................................................53 Part 2. The Eucaryotic CellGeneral Information..........................................................................................58 Experimental Analysis III 8. Enzyme Cytochemistry....................................................................... 59 9. Analysis of a Membrane Receptor...................................................... 59 10. The Cell Nucleus................................................................................. 86 Part 3. Nucleic AcidsI. Nucleic Acid Structure and Function: A Review of the Basics.........................107 II. Electrophoretic Analysis of DNA General Information........................................................................................118 Experimental Analysis IV 11. The Length of DNA Molecules...........................................................122 12. Restriction Nuclease Mapping of DNA...............................................126 13. Plasmid DNA Structure.......................................................................132 14. The Nucleosome Structure of Chromatin............................................136
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