Modern
Biology Inc
Customer Information and Ordering:
1-800-733-6544
|
|
|
Search for: Search by Your Laboratory Class
|
This Program is a complete laboratory course for teaching molecular biology or biochemistry at the advanced college level. The program is designed for up to 16 students working in pairs and provides essentially all of the chemicals and instructions that are needed to teach twenty eight 2-3 hour laboratory sessions. The course consists of experiments carefully selected from the Standard Programs that are presented in a comprehensive integrated laboratory manual. The Table of Contents of the manual and the catalog numbers of the experiments provided with the program are shown below. To use this program, you will need an Accessory Kit, the appropriate electrophoresis equipment, microscopes, a water bath for DNA hybridization, and a centrifuge that can be operated at a force of at least 3000 x g. A bacterial shaker is also strongly recommended. The price of the course is about $77 per student per semester if these items are available in your teaching laboratory.In this laboratory course, your students will be introduced to many of the concepts and techniques that have revolutionized the biological sciences during the past two decades. In the first section of the course, students study topics in protein biology and biochemistry such as protein structure, function, isolation, location in cells, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis (both native and denaturing), affinity chromatography, peptide mapping, enzyme cytochemistry and the Western blot procedure. In the second section of the course, students perform experiments with DNA structure and function. These exercises stress the organization and complexity of the prokaryotic and eukaryotic genomes, gene function and regulation, and the structure of the eukaryotic chromosome. Techniques include restriction nuclease mapping, Southern blot hybridization procedures, and an array of state-of-the-art DNA cloning techniques. Features
The experiments that comprise this course were selected from six programs. Program Experiments Standard 1 101, 102, 106 Standard 2 201, 204, 205, 206 Standard 3 301, 302, 303, 306 Standard 4 401, 403 Standard 5 Entire Program Standard 7 701, 702, 703 Price List-Laboratory Course 4
Table of Contents Part 1. The Proteins I. Protein Composition and Structure: A Review of the Basics.............................. 1 II. Electrophoresis-General Information and Analysis of Native Proteins Theoretical Aspects.......................................................................................... 7 Practical Aspects and Procedures.................................................................... 11 Experimental Analysis I 1. Electrophoretic Separation of Proteins................................................ 17 2. Genetics and Sickle Cell Anemia........................................................ 20 3. LDH Isoenzymes................................................................................. 26 III. Electrophoretic Analysis of Denatured Proteins General Information....................................................................................... 32 Experimental Analysis II 4. Molecular Weight Determination........................................................ 36 5. Peptide Mapping Analysis.................................................................. 40 6. Protein Evolution and the Western Blot.............................................. 44 7. Affinity Chromatography.................................................................... 53 Part 2. The Eucaryotic CellGeneral Information................................................................................ 58 Experimental Analysis III 8. Enzyme Cytochemistry....................................................................... 59 9. Analysis of a Membrane Receptor...................................................... 74 10. The Cell Nucleus................................................................................. 86 Part 3. Nucleic AcidsI. Nucleic Acid Structure and Function: A Review of the Basics....................... 107 II. Electrophoretic Analysis of DNA General Information..................................................................................... 118 Experimental Analysis IV 11. The Length of DNA Molecules......................................................... 122 12. Restriction Nuclease Mapping of DNA............................................ 126 13. Plasmid DNA Structure..................................................................... 132 14. The Nucleosome Structure of Chromatin.......................................... 136 III. DNA Hybridization Analysis General Information..................................................................................... 148 Experimental Analysis V 15. Evolution of the Vertebrate Genome................................................. 161 16. Identifying a Specific Sequence in the Mammalian Genome........... 171 Part 4. Genetic EngineeringGeneral Information.......................................................................... 186 Experimental Analysis VI 17. Construction of a Recombinant DNA Library.................................. 191 18. Transformation of E. coli.................................................................. 202 19. Isolation of Plasmid DNA................................................................. 207 20. Mapping of Plasmid DNA................................................................. 213 21. Analysis of Plasmid DNA by Southern Blot Hybridization.............. 216 |
Send mail to
Jeff@modernbio.com with questions
or comments about this web site or Modern Biology Inc.
|
