Modern
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This Program is a complete laboratory course for teaching cell and molecular biology at the college level. The program is designed for 16 students working in pairs and provides essentially all of the chemicals and instructions that are needed to teach sixteen 3 hour laboratory sessions. The course consists of 16 experiments carefully selected from 7 Laboratory Programs that are presented in a comprehensive integrated laboratory manuals. The Table of Contents of the manual and the catalog numbers of the experiments provided with the program are shown below. To use this program, you will need an Accessory Kit, the appropriate electrophoresis equipment, microscopes, a water bath, and a small centrifuge such as tabletop model or microcentrifuge. The price is about $52 per student per semester if these items are available in your teaching laboratory. In this laboratory course, college freshman and sophomore-level students will be introduced to the molecular biology of the eukaryotic cell. In the first two sections of the course, students study topics in protein biology and biochemistry such as protein structure, function, molecular evolution, and the detection and molecular basis of human disease. Techniques used for these experiments include electrophoresis and the Western blot procedure. In the third section of the course, students localize enzymes in plant and animal cells and study the properties of a specific cell-surface receptor. Students perform a project of their own design in the fourth section of the course. The projects focus on the characterization of plant peroxidases. For the projects, assorted vegetables or plants are needed but not included. Experiments on the properties and structure of DNA are presented in the final section of the course. Here, students perform experiments that deal with genome organization, chromatin structure, and specific gene function. Techniques include DNA electrophoresis, cell fractionation, DNA isolation, restriction nuclease mapping, and basic cloning procedures. Features
The experiments that comprise this course were selected from 7 Programs. Program Experiment Basic 1 1, 2 Basic 4 2 Standard 1 101, 102 Standard 3 306 Standard 7 701, 702 Standard 8 801, 803, 804 Standard 10 1001, 1002, 1004 Others IND-2, IND-5 Descriptions of the experiments and chemicals are given under the individual program descriptions. Price List - Laboratory Course 5
Table of Contents Experimental Analysis I: Protein Structure and Evolution Protein Composition and Structure: A Review of the Basics.................... 1 Electrophoresis-General Information and Analysis of Native Proteins Theoretical Aspects.......................................................................... 7 Practical Aspects and Procedures....................................................... 11 1. Electrophoretic Separation of Proteins............................................. 17 2. Genetics and Sickle Cell Anemia..................................................... 20 3. Serum Proteins and the Western Press-Blot..................................... 26 Experimental Analysis II: Protein Function 4. Specificity of Albumin Binding........................................................ 38 5. Extraction and Analysis of an Enzyme from Wheat............................ 47 6. Tissue-Specific Isoenzymes in the Cow............................................ 59 Experimental Analysis III: Location of Proteins in Cells 7. Enzyme Cytochemistry................................................................... 66 8. Analysis of a Cell Surface Receptor.................................................. 81 Experimental Analysis IV: Characterization of Peroxidase in Plants (Student Designed Projects) Description........................................................................................ 93 9. Tissue Printing............................................................................... 94 10. Peroxidase Isoenzymes................................................................. 106 Experimental Analysis V: DNA and the Cell Nucleus Nucleic Acid Structure and Function: A Review of the Basics..................... 117 Electrophoretic Analysis of DNA 11. Anatomy and Evolution of the Genome............................................. 130 12. Properties of DNA......................................................................... 141 13. Analysis of a Genomic Segment ..................................................... 146 14. Cell Fractionation and DNA Isolation............................................... 154 15. Genotype to Phenotype................................................................. 161 16. The Nucleosome Structure of Chromatin.......................................... 173
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